Transposon Mutagenesis Identifies Uropathogenic Escherichia coli Biofilm Factors

Transposon mutagenesis identifies uropathogenic Escherichia coli biofilm factors.

Uropathogenic Escherichia coli (UPEC), which accounts for 85% of urinary tract infections (UTI), assembles biofilms in diverse environments, including the host. Besides forming biofilms on biotic surfaces and catheters, UPEC has evolved an intracellular pathogenic cascade that culminates in the formation of biofilm-like intracellular bacterial communities (IBCs) within bladder epithelial cells....

Zinc oxide nanoparticle reduced biofilm formation and antigen 43 expressions in uropathogenic Escherichia coli

Objective(s): This study aimed to investigate the effect of zinc oxide nanoparticles (ZnO-np) on biofilm formation and expression of the flu gene in uropathogenic Escherichia coli (UPEC) strains. Materials and Methods: Minimum inhibitory concentration (MIC) of ZnO-np was determined by agar dilution method. The effect of MIC and sub-MIC concentrations of ZnO-np on biofilm formation were determin...

Fimbriae of Uropathogenic Escherichia coli Mediate Biofilm Formation

Uropathogenic Escherichia coli (UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate attachment to specific receptors, enhance persistence and trigger innate host responses. UPEC produce a range of fimbrial adhesins, with type 1 and P fimbriae of the chaperone-usher subclass b...

Biofilm Formation in Uropathogenic Escherichia coli Strains: Relationship with Urovirulence Factors and Antimicrobial Resistance

Transposon Mutagenesis Screen Identifies Potential Lung Cancer

40 Non-small cell lung cancers (NSCLCs) harbor thousands of passenger events that hide genetic 41 drivers. Even highly recurrent events in NSCLC, such as mutations in PTEN, EGFR, KRAS, and ALK, 42 are only detected in, at most, 30% of patients. Thus, many unidentified low-penetrant events are causing 43 a significant portion of lung cancers. To detect low-penetrance drivers of NSCLC a forward g...